Journal: Journal of hazardous materials

Article Title: In Vitro characterization of the endocrine disrupting effects of per- and poly-fluoroalkyl substances (PFASs) on the human androgen receptor

doi: 10.1016/j.jhazmat.2022.128243

Figure Lengend Snippet: Antiandrogenic effect of PFASs on the expression of (A) PSA , (B) FKBP5, and (C) AR in VCaP human prostate cancer cells. Cells were cotreated with 625 pM testosterone and PFASs or 50 nM OHF (positive control) for 24 h under hormone deprived conditions (10% CD FBS). Values are expressed as the mean fold change ± S.E.M. from three independent experiments. Statistical analysis was performed using one-way ANOVA followed by a multiple comparison analysis with Dunnett’s test to compare the difference in fold change between exposed groups and the corresponding control (625 pM testosterone). Significance levels are represented with asterisk as following: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. The P trend is determined based on linear regression analysis.

Article Snippet: The human prostate cancer cell line (VCaP) which expresses high levels of wild-type AR, was purchased from ATCC.

Techniques: Expressing, Positive Control, Comparison, Control

Journal: Journal of hazardous materials

Article Title: In Vitro characterization of the endocrine disrupting effects of per- and poly-fluoroalkyl substances (PFASs) on the human androgen receptor

doi: 10.1016/j.jhazmat.2022.128243

Figure Lengend Snippet: Antiandrogenic effect of PFASs on the AR protein levels in prostate cancer cells (VCaP). (A and B) cells were cotreated with 625 pM testosterone (T) and PFASs or 50 nM OHF (positive control) for 24 h under hormone deprived conditions (10% CD FBS). (C and D) cells were treated with PFASs or 1 μM enzalutamide (Enz, positive control) for 48 h under normal conditions (containing 10% intact, non-CD stripped FBS). AR proteins were detected using AR-specific antibody and α-tubulin was used as a loading control. Values are expressed as the mean fold change ± S.E.M. from three independent experiments. Statistical analysis was performed using one-way ANOVA followed by a multiple comparison analysis with Dunnett’s test to compare the difference in fold change between exposed groups and the corresponding control (625 pM testosterone for panel B) and (0.1% v/v DMSO for panel D). Significance levels are represented with asterisk as following: *p < 0.05 and * **p < 0.001.

Article Snippet: The human prostate cancer cell line (VCaP) which expresses high levels of wild-type AR, was purchased from ATCC.

Techniques: Positive Control, Control, Comparison